What is it? Why is it important?
Prior to analysis, collected Biological Material (BM) is stored for a given period of time. Based on required storage temperature, different storage options exist:
- Cabinets (room temperature)
- Fridge (2°C to 10°C)
- Freezer (-35 to -18°C)
- Ultra-freezer (-85 to -60°C)
- Cryogenic storage (< -135 °C)
Safeguarding the quality and integrity of BM, requires that storage equipment is continuously supervised. Storage equipment should be:
- Serviced to ensure optimal performance
- Protected against unauthorized access (e.g. prevents unacceptable handling of storage equipment resulting in damage or loss of BM)
- Monitored on an ongoing basis with 24hours / 7 days a week surveillance (e.g. temperature- and humidity control, protected from light exposure)
- Equipped with emergency procedures in the event required storage conditions deviate (e.g. a drop or rise in temperature)
Large biobanks (>2 million samples), have automated storage systems. Robots take over sample storage and retrieval processes. Even though quite costly, these solutions allow for efficient and high quality storage of BM (e.g. rapid error-free BM storage and retrieval).
What do I need to do?
- Storage conditions (e.g. temperature / humidity control)
- Infrastructure (e.g. access protected premises, integration into the local alarm logistics)
- Storage guidelines for infectious (e.g. HIV / HCV), radioactive, or genetically modified BM
- Criteria towards equipment manufacturers (e.g. minimum attainable temperature, temperature stability, available storage space, inbuilt alarm, locking system, cost)
Before activating an equipment:
- Add the apparatus to your equipment inventory (e.g. labeled with an identifier number)
- Write an SOP/WI and define:
- A maintenance plan (e.g. add a logbook that tracks equipment maintenance history)
- An emergency plan (e.g. implemented in the event of equipment failure)
- Access guidelines (e.g. restrict access to storage and removal of BM to biobanking staff)
- Train biobanking staff on equipment SOP/WI
Additional questions to ask when buying new storage equipment:
- What is the storage capacity / volume?
- How is the storage content organized (e.g. number of drawers or shelves, type of racks)?
- What is the expected temperature stability (e.g. expected maximum range when in operation)?
- What is the temperature recovery performance after opening / closing storage doors?
- Equipment footprint (e.g. chest vs. shelf freezer)?
- Level of automation (e.g. robot support)
- What is the price performance ratio?
Where can I get help?
Your local CTU↧ can support you with experienced staff regarding this topic
Basel, Departement Klinische Forschung, CTU, dkf.unibas.ch
Lugano, Clinical Trials Unit, CTU-EOC, www.ctueoc.ch
Bern, Clinical Trials Unit, CTU, www.ctu.unibe.ch
Geneva, Clinical Research Center, CRC, crc.hug.ch
Lausanne, Clinical Research Center, CRC, www.chuv.ch
St. Gallen, Clinical Trials Unit, CTU, www.kssg.ch
Zürich, Clinical Trials Center, CTC, www.usz.ch
The Swiss Biobanking Platform (SBP) can provide you with support on this topic.
SOPs, Forms and Templates – see in particular
- Equipment management SOP
- Equipment Inventory
ISO 20387:2018 Biotechnology - Biobanking (access liable to cost) - General Requirements for Biobanking – see in particular sections
- 6.5 Equipment
- 7.7 Storage of biological material